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Literature summary extracted from

  • Vigetti, D.; Deleonibus, S.; Moretto, P.; Karousou, E.; Viola, M.; Bartolini, B.; Hascall, V.C.; Tammi, M.; De Luca, G.; Passi, A.
    Role of UDP-N-acetylglucosamine (GlcNAc) and O-GlcNAcylation of hyaluronan synthase 2 in the control of chondroitin sulfate and hyaluronan synthesis (2012), J. Biol. Chem., 287, 35544-35555.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.212 additional information HAS2 can be O-GlcNAcylated on serine 221, which strongly increases its activity and its stability to half-life of over 5 h versus 17 min without O-GlcNAcylation Homo sapiens
2.4.1.212 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate increases O-GlcNAcylation and HA synthesis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 stable expression of N-terminally Myc-tagged human HAS2 in NIH3T3 cells membranes, the molecular mechanism that underlies the rapid c-Myc-HAS2 turnover involves the 26 S proteasome, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.212 S221A site-directed mutation of the O-GlcNAcylable Ser-221 to alanine generated an enzyme with a calculated t1/2 of about 70 min Homo sapiens

General Stability

EC Number General Stability Organism
2.4.1.212 HAS2 can be O-GlcNAcylated on serine 221, which strongly increases its activity and its stability to half-life of over 5 h versus 17 min without O-GlcNAcylation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 microsome
-
 Homo sapiens
-
-
2.4.1.212 plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.212 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.212 additional information Homo sapiens HAS2, localized in the plasma membrane, uses cytoplasmic UDP-glucuronic acid and UDP-N-acetylglucosamine as substrates ?
-
 ?
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] Homo sapiens
-
 UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] Homo sapiens
-
 UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
 isozyme HAS2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.212 additional information HAS2 can be O-GlcNAcylated on serine 221, which strongly increases its activity and its stability to half-life of over 5 h versus 17 min without O-GlcNAcylation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 aortic smooth muscle cell HAS2 is the main synthase in aortic smooth muscle cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 additional information HAS2, localized in the plasma membrane, uses cytoplasmic UDP-glucuronic acid and UDP-N-acetylglucosamine as substrates Homo sapiens ?
-
 ?
2.4.1.212 UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
-
 Homo sapiens UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
-
 ?
2.4.1.212 UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan]
-
 Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 Has2
-
 Homo sapiens
2.4.1.212 hyaluronan synthase 2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.212 metabolism regulation of hexosamine biosynthetic pathway, biosynthesis of hyaluronan and other glycoconjugates, and protein O-GlcNAcylation, overview Homo sapiens
2.4.1.212 physiological function the role of UDP-N-acetylglucosamine and O-GlcNAc-acylation of hyaluronan synthase 2 in the control of chondroitin sulfate and hyaluronan synthesis, overview. O-linked GlcNAc (O-GlcNAcylation) is regulated by the action of two enzymes, O-GlcNAc transferase and O-GlcNAc hydrolase. The HA increase due to O-GlcNAcylation regulates inflammatory cell adhesion, the number of monocytes that adhere on AoSMC monolayer cultures is increased Homo sapiens